PNC27 -10 vials

Biological Activity

Purity & Documentation

References

Customer Review

Description

PNC-27, a chimeric p53-penetratin peptide binds to HDM-2 in a p53 peptide-like structure, induces selective membrane-pore formation and leads to cancer 
cell lysis
. PNC-27 is an 
anticancer
 peptide. PNC-27 can be used in acute myeloid leukemia research
1
2
3
.

In Vitro

PNC-27 (50 μg/mL; 0-3 h) induces 
cancer
 cell death
1
.

PNC-27 (50 μg/mL; 15 min) binds to cell membrane-bound HDM-2 in A2058 and MCF-7 cells
1
.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PNC-27 Related Antibodies

p53 Antibody

Acetyl-p53 (Lys370) Antibody (YA640)

Phospho-p53 (Ser37) Antibody

Phospho-p53 (Ser6) Antibody (YA166)

FOXO4 Antibody (YA426)

p53 Antibody (YA250)

p53 (acetyl K382) Antibody (YA251)

p53 DINP1 Antibody (YA249)

p53 (acetyl K370)Antibody (YA248)

Phospho-p53 (Ser392) Antibody (YA167)

p21 Antibody (YA254)

PERP Antibody (YA1674)

Phospho-MDM2 (Ser166) Antibody (YA1888)

p73 Antibody (YA1240)

PEPD Antibody (YA1578)

IFRD1 Antibody (YA2507)

TP53BP2 Antibody (YA2576)

Cell Cytotoxicity Assay
1

Cell Line:

MIA-PaCa-2 cells

Concentration:

50 μg/mL

Incubation Time:

0-3 hours

Result:

Induced 100% cell death in 90 min.

In Vivo

PNC-27 (intraperitoneal injection; 40 mg/kg; once daily; 2-3 w) shows anti-leukemia activity in mice
2
.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mll
PTD/WT
/
Flt3
ITD/ITD
 AML mice
2

Dosage:

40 mg/kg

Administration:

Intraperitoneal injection; 40 mg/kg; once daily; 2 or 3 weeks

Result:

Reduced AML engraftment and prolonged survival.

Molecular Weight

4031.73

Formula

C
188
H
293
N
53
O
44
S

CAS No.

1159861-00-3

Appearance

Solid

Color

White to off-white

Sequence Shortening

PPLSQETFSDLWKLLKKWKMRRNQFWVKVQRG

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder

-80°C

2 years

-20°C

1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H
2
O : ≥ 100 mg/mL
 (
24.80 mM
)

*
“≥” means soluble, but saturation unknown.

Preparing

Stock Solutions

Concentration
Solvent
Mass

1 mg

5 mg

10 mg

1 mM

0.2480 mL

1.2402 mL

2.4803 mL

5 mM

0.0496 mL

0.2480 mL

0.4961 mL

 View the Complete Stock Solution Preparation Table

*
 Please refer to the solubility information to select the appropriate solvent. Once prepared, 
please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles
.

Storage method and period of stock solution: 
-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

*
Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.